This article covers exactly how receptor downregulation occurs with daily dosing, what cycle structures preserve sensitivity, and what dosing mistakes render the peptide ineffective halfway through a protocol
Tags can (a) change protein stability (b) protein conformation (c) inhibition of enzymatic activity (d) alterations in biological function/activity (d) undesired changes in protein structure for structural studies (e) toxicity
DAgostino, I., Tacconelli, S., Bruno, A., Contursi, A., Mucci, L., Hu, X., Xie, Y., Chakraborty, R., Jain, K., Sacco, A., Zucchelli, M., Landolfi, R., Dovizio, M., Falcone, L., Ballerini, P., Hwa, J., & Patrignani, P
Tim-3 induces apoptosis in Thl and cytotoxic T cells and regulates the function of NK cells, NKT cells and macrophages
Liu Q, Wang S, Cai L
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